8kb cdna with 1362bp orf and codes 454 amino acids with only a sh2 domain . the gene was named as sh2a at chromosome 8p22 . we study its structure by blast homologous analysis and its expression by rt - pcr and northern blot 本文将通过蛋白质分析软件研究其结构和同源性，利用rt - pcr和northern印迹杂交技术研究该基因的表达情况及该基因与肿瘤的关系。
Northern blot analysis shows that a 3 . 2 kb transcript specific for znf323 was expressed at high levels in the lung , liver , and kidney , while weakly expressed in intestine , brain , muscle , cholecyst , heart , and pancreas Northernbolt分析表明znf323基因在人类早期胚胎不同时期( 18 25周)表达水平不同，在肺，肾和肝脏的表达水平较强，其它组织表达较弱。
4 northern blotting showed that the fe ( ii ) - transporter mrna was expressed lower in fe - sufficiency roots and was strengthened expression by the treatment of fe - deficiency , particularly in the sixth day of the treatment in roots 3 、 southern杂交分析表明： fe ( )转运蛋白基因在小金海棠基因组中可能是单拷贝；同时证明山定子和珠眉海棠基因组dna中也存在类似基因。
Northern blot analysis showed that the mrna level of p27 k ' pl had no changes during ir responses ; the level of ubiquitinated p27klpl increased following 10gy - irradiation ; mg - 132 ( proteasome inhibitor ) inhibited the degradation of 我们通过初步的研究结果揭示: northem检测结果表明ir对p27kip ‘ mrna水平无明显影响; ir诱导p27kip ’泛素化水平的增加;蛋白酶体抑制剂( mg一1
Northern blot analysis showed higher expression level of pds gene in the stigma and anther than in the leaf and stem . the information we have got is very important for the further research of gene structure , expression and function of phytoene desaturase 此基因的克隆，为深入研究八氢番茄红素脱氢酶基因结构、表达和调节机制及其结构和功能的关系奠定了重要的基础。
Northern blot hybridization showed that leerfl , leerf2 and pti4 ( a member of erebps in tomato ) expressed differentially in different parts of wild tomato fruit during ripening , which was related with fruit ripening Northern杂交分析表明： leerf1 、 leerf2 、 pti4 (已发表的番茄erebps成员)基因在普通番茄果实的成熟过程及果实的不同部位都有差异表达，表现出与成熟进程的相关性。
Northern blot and rt - pcr analysis show that sh2a gene is ubiquitously expressed in many tissues with three transcripts . the aberrant expression of sh2a gene in some cancers was found . it suggests that sh2a gene relates to tumors Rtpcr及northern印迹杂交发现sh人在多种组织中有表?二?达，有3个转录本，而且在癌及癌旁组织的比较中发现，肿瘤组织中表达较高，初步证明与肿瘤的发生有关。
Whether the gene was transformed into the arabidopsis was confirmed by pcr - southern blotting , southern blotting and northern blotting . 1 . pcr - southern blotting showed that all kanamycin - tolerant plants had strong positive signals , and no signal was shown in wild type plants Pcr结果扩增出1 . 4kb的特异性条带，表明sod2已整合进拟南芥中， pcr - southern进一步证实了pcr结果的正确性。
Northern blot suggested that the transcription of nramp gene is induced by iron stress in both roots and leaves and strengthened by iron stress in leaves ; the transcription of irt gene can be induced and strengthened by iron stress in roots Northern杂交结果表明：在根中和叶中， nramp基因均能得以转录且受缺铁胁迫的诱导而加强。 fe ( ) -转运蛋白基因在根中的转录也受到缺铁胁迫的诱导，并随缺铁胁迫处理天数的增加而加强。
Some transgenic plants harboring the gfp tnos vector also exhibited inhibited expression of gfp , and northern blots also revealed that most gfp mrnas transcribed from tnos accumulated in the nucleus and was not transported into the cytoplasm 获得了含有gfp - tnos表达载体的转基因本生烟，在部分转基因株系中gfp基因的表达受到抑制。 northern分析显示： gfp - tnos载体所表达的gfp基因产物多数滞留在细胞核内部，不能被运送到细胞质中。
